Pritish Ranjan

5 Questions 13 Answers 0 Followers

Questions related from Pritish Ranjan

Can LB agar plates be used for disc diffusion assay instead of Mueller Hinton agar?

Can anyone let me know if i could use LB agar plates instead of Mueller Hinton agar plates. If possible then provide me some standard references also. Thank you

14 July 2016 4,128 3 View

What is the relation between OD600 and CFU/ml of Mycobacterium smegmatis?

I am working on M. smegmatis, and i want to calculate the CFU/ml concentration for the cell growth i obtain using the OD at 600 nm. Kindly tell me what is the corresponding value of CFU/ml for 0.1...

14 February 2016 6,476 6 View

MAximum load till which Ampicillin will show bactericidal activity?

I am using S.aureus . Can anyone tell me till what would be the maximum initial microbial load at which Ampicillin will show bactericidal activity?

31 January 2016 3,616 8 View

Can I use TCA precipitation to concentrate proteins for SDS PAGE ?

I want to concentrate my proteins using TCA, and do 1-D SDS PAGE. I have a few concenrns. i) Will the TCA remove salts or concentrate them as well ii) For resoulubilizing precipitated proteins, is...

02 July 2015 1,323 13 View

How should I choose between western blots of protein and paraformaldehyde fixed cells to probe with antibodies?

When the primary-secondary antibodies are used to identify proteins using HRP or alexafluor, how to make a choice whether i should extract the proteins, perform electrophoresis and blotting and...

19 May 2015 7,683 2 View