Pritish Ranjan

7 Questions 15 Answers 0 Followers

Questions related from Pritish Ranjan

Can LB agar plates be used for disc diffusion assay instead of Mueller Hinton agar?

Can anyone let me know if i could use LB agar plates instead of Mueller Hinton agar plates. If possible then provide me some standard references also. Thank you

14 July 2016 4,184 3 View

What is the relation between OD600 and CFU/ml of Mycobacterium smegmatis?

I am working on M. smegmatis, and i want to calculate the CFU/ml concentration for the cell growth i obtain using the OD at 600 nm. Kindly tell me what is the corresponding value of CFU/ml for 0.1...

14 February 2016 6,567 6 View

MAximum load till which Ampicillin will show bactericidal activity?

I am using S.aureus . Can anyone tell me till what would be the maximum initial microbial load at which Ampicillin will show bactericidal activity?

31 January 2016 3,659 8 View

How can I quantify B. subtilis growth in liquid culture using absorption at 600 nm?

I am growing B. subtilis in liquid culture, i see biofilm formation in 10-12 hours,. If i want to quantify cell growth, how should i do it using OD at 600nm? Since a lot of cells are a part of the...

02 December 2015 3,067 1 View

How can I distinguish Bacteriocidal activity from Bacteriostatic activity of any antimicrobial compound?

If i wanted to test whether a given antimicrobial is showing bacteriocidal activity or bacteriostatic activity, which tests i would need to perform? Which techniques will it involve and how...

27 November 2015 2,109 5 View

Can I use TCA precipitation to concentrate proteins for SDS PAGE ?

I want to concentrate my proteins using TCA, and do 1-D SDS PAGE. I have a few concenrns. i) Will the TCA remove salts or concentrate them as well ii) For resoulubilizing precipitated proteins, is...

02 July 2015 1,370 13 View

How should I choose between western blots of protein and paraformaldehyde fixed cells to probe with antibodies?

When the primary-secondary antibodies are used to identify proteins using HRP or alexafluor, how to make a choice whether i should extract the proteins, perform electrophoresis and blotting and...

19 May 2015 7,736 2 View