Prisca Mbikou

2 Questions 7 Answers 0 Followers

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Troubleshoot Isolated heart langendorff system: how to get rid of ectopic heartbeat and signal?

Hi there, I am working on rat's isolated hear using langendorff system. I apply a specific heart rate of 310 bpm and measure parameters such as: Left ventricle pressure (LVP), perfusion pressure...

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How do I quantify the phosphorylation relative to total protein on western blot?

I am working on AKT phosphor and tot protein. I have blots with both proteins and need to quantify the phophoAKT band relatively to the total AKT band, both bands being detected by 2 different...

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