Princy Merlin Mathew

3 Questions 4 Answers 0 Followers

Questions related from Princy Merlin Mathew

To improve transfection efficiency in MDA231cells?

I am trying to transfect MDA 231 cells but my efficiency is too low. I tried up to 2ug plasmid (7.5KB) in 12 well plate with different transfection reagents (PEI and Lipofectamine 3000) For PEI i...

19 July 2018 2,893 3 View

What is the problem with my siRNA transfection?

I tried siRNA transfection against NAK ATPase gene in breast cancer cells using various reagents but it all failed. I did it with transfection reagents like lipofectamine for RNAiMAX, Stemfect...

27 April 2013 6,956 13 View

How to get rid of unhybridised RNA from a hybridized reaction mix?

I did invitro transcription from a PCR template. The invitro transcribed mRNA was hybridized with an oligo. Am I supposed to transfect this hybridized oligo into the cells but I have to get rid of...

24 April 2013 275 4 View