MSCV LTR plasmid vs AAV (pCMV promoter) plasmid transfections for CRISPRa?

I have been trying to transfect primary mouse neural progenitor cells (wildtype and heterozygous for our gene of interest) with a plasmid expressing dCas9-VP64 and a plasmid expressing sgRNA for...

26 February 2025 7,693 1 View