Prerna Sharma

3 Questions 3 Answers 0 Followers

Questions related from Prerna Sharma

Problem with western blot of ABCB1 from mice colon tissue ?

I am getting a problem in western blot for ABCB1 in colon tissue in mice. The protein ABCB1 is a drug transporter 170kDa. I am using 8% SDS gel and 0.2um nitrocellulose membrane. The expression...

24 April 2017 1,129 2 View

What is the best method for extraction of RNA from adipose tissue ?

The lipids in adipose tissue interfere with the extraction of RNA via standard Trizol method. The lipid interference is preventing the pellet formation in the Trizol method. Please suggest method...

07 April 2016 6,571 3 View

How do I remove collagens from my collagenase-digested tissue solution when the adipocytes don't settle down?

I am facing a problem in isolatng adipocytes from rat epididymal fat pads. By following the Rodbell method, in which fraction are the adipocytes present? At the last step, after centrifugation of...

16 October 2014 2,252 8 View