Praveen Kumar Raju

6 Questions 7 Answers 0 Followers

Questions related from Praveen Kumar Raju

Does 2% Triton-X treatment in co-immunoprecipitation also affect the protein interaction?

I am using 2% Triton-X to wash the non-specific binding of protein to the beads in a Co-IP experiment. But I am not able to see any protein band of my choice when I run a gel. Is it possible that...

11 November 2013 10,010 8 View

Synchronization of SH-SY5Y Neuroblastoma cells

Hi, I am working with SH-SY5Y cell line and if possible I want to synchronize the population at G1/S boundary and release them in order to enrich the dividing cells, much the way done for any...

09 September 2013 531 1 View

Does anyone have experience with FRAP in live cells?

I am performing FRAP in live cells to check the dynamics of my interested protein tagged with GFP. Its DNA binding protein. But, when I expose the sample, the entire area bleaches even if I point...

07 July 2013 6,449 2 View

Serum starvation of NIH3T3 cells?

What 's the minimum time frame (in days/hours) required for serum starvation in NIH3T3 cells to induce quiescence?

04 April 2013 3,129 10 View

Is it possible to differentiate human neuroblastoma cell line SHSY-5Y to form neurite using low serum concentration?

10 October 2012 1,758 6 View

What is the minimum time duration of serum starvation in NIH3T3 cells for cilia formation?

Hi everyone, can some body help me in figuring out : What is the minimum time duration for serum starvation of NIH3T3 cells to induce cilia formation ?

09 September 2012 7,277 1 View