Pratik Patil

2 Questions 9 Answers 0 Followers

Questions related from Pratik Patil

Is anybody familiar with the quantification of nucleotides (GTP/ATP/AMP/pppGpp)?

I am working with a protein that uses GTP and ATP as substrates and form hyperphosphorylated GTP and AMP as products. So to monitor the reaction I want an assay, (other than radioactive assay)...

09 September 2014 1,726 4 View

What is the minimum stirring speed (rpm) that can be used in microcal ITC?

I am doing nucleotide binding experiments for my protein, using microcal ITC. The experiment is done at 30 degree C. with stirring speed of 500/300 rpm. Every time I find that protein precipitates...

07 July 2014 5,002 9 View