Prateek Sharma

4 Questions 5 Answers 0 Followers

Questions related from Prateek Sharma

Do anyone use RNA-ZOL for miRNA isolation? What is its efficacy and how does it works?

I want to isolate miRNA manually. Till now I have used kits for the same purpose. Recently I heard about RNA-ZOL, but have not found any article citing its use. Do anyone use it? .

09 July 2014 1,636 1 View

Why do cells lines like T47D or MCF 7 derived from metastatic sites (e.g. pleural effusion) remain non-metastatic?

07 May 2014 7,036 0 View

What happens to cell lines if they are transferred from liquid nitrogen to minus 80 degrees. Are they still viable?

I want to know if the low temperature can change the viability of cell lines.

05 October 2013 3,384 7 View

Is it possible to extract miRNAs manually from cells? If yes. then what is the protocol to isolate them?

I want to isolate miRNA.

05 October 2013 3,791 2 View