Prashanth Anamthathmakula

4 Questions 8 Answers 0 Followers

Questions related from Prashanth Anamthathmakula

What bioinformatic tools can I use to identify probable hnRNP regulatory sites in mouse GOI mRNA?

I am looking at alternative splicing in mouse mRNA and would like to identify if there are any heterogeneous nuclear ribonucleoproteins (hnRNPs) binding sites that influence the splicing events...

05 December 2018 4,419 2 View

Chip-qPCR to differentiate regions that are 100bp apart?

Hi. I am doing chip-qpcr and have analyzed the region using specific primers for the GOI. The sonication has given me sheared DNA in the range of 700 bp to 2.5 kb size. I have 4 different regions...

16 April 2018 2,626 6 View

Cloning custom shRNA sequence into pGIPZ plasmid?

Is it possible to replace an existing shRNA sequence with a custom shRNA sequence into the pGIPZ vector ? If the sequence is right, then the only way to test is through lentivirus, because the...

15 June 2016 1,539 5 View

Why 6X His sequence is not being detected by anti-6X his antibody in a western blot?

I am over expressing my protein which has a 6x his tag, using lentiviral infection in a mammalian cell line. I also have a reporter in my lentiviral construct which gives me an idea of...

13 June 2016 9,495 6 View