2 Questions 6 Answers 0 Followers
Questions related from Prashant Mudaliar
I would like to know about the inhibition of autophagy by wortmannin: i) For how long should cells be incubated with wortmannin and for how long does it inhibits autophagy? ii) Is Beclin-1 siRNa...
08 August 2013 8,713 1 View
I am finding it difficult to make stable cells expressing GFP LC3 ( U-87 MG , IMR-32). After 24 hrs of transfection 80% of cells tend to lose expression. And within the next 24 hours, 95% cells...
10 October 2012 3,764 1 View