Prasanth Francis

4 Questions 3 Answers 0 Followers

Questions related from Prasanth Francis

Can MTT assay results indicate probable apoptosis?

I tested some synthesized molecules for antiproliferative activity on MDA MB231, HCT 116, MCF-7 and HT 29 cell lines using MTT assay. They all showed good activity in nano molar range. Can the MTT...

11 March 2017 7,445 2 View

During flow cytometric apoptosis data analysis, what does it mean if the population of normal cells increase after a time period?

I performed a flow cytometric analysis of apoptosis induced by synthesized compounds. i used 3 concentrations of single compound. each set incubated for 24h and 48h. i could easily figure out a...

03 March 2016 5,813 3 View

Please suggest a protocol for performing Annexin V FITC/PI staining assay on Hela cells

I am trying to perform Annexin V FITC/PI staining on hela cells to study apoptotic effect of some synthesised compounds. I'am using BD bioscience kit and they have protocol for adherent cells...

23 January 2016 8,900 2 View

How can I evaluate Haspin kinase inhibitory activity of a chemical entity?

I have synthesized some compounds which were designed as Haspin kinase inhibitors. I have checked the IC 50 values in MDAMB-231 cell lines. Best active compounds showed IC 50 Values 17 µg/mland...

26 July 2015 8,922 1 View