Pranita Patil

4 Questions 2 Answers 0 Followers

Questions related from Pranita Patil

Why the cloning of the promoter region from genomic DNA is failing?

I amplified the promoter region from genomic DNA, and then I extracted the specific band using gel. I also checked by loading it onto a gel again, and then I did blunt end ligation in PJET. I got...

11 December 2024 8,094 1 View

How to deal with missing X,Y,Z co-ordinates of particular atom in ligand .cif file ?

I am trying to prepare parameter files for G7M modified residue for MD simulation using Amber. I got G7M.cif from RCSB PDB but in that file co-ordinates for H0P3 are missing which is causing an...

08 October 2019 7,956 2 View

Can you give me any suggestions regarding targeted MD simulation?

I want to targeted MD simulation of macromolecule using AMBER software .As i am quite new to MD simulation field and of such a big complex .

23 July 2019 9,372 1 View

How to confirm the RNA sequence for any mutation after in vitro transcription?

I have to do in vitro transcription of ssDNA then PCR and using PCR product for transcription by T7 polymerase of repeat containing RNA.So after purification and denaturing gel electrophoresis ,...

01 June 2019 3,339 2 View