Pranav Seth

5 Questions 4 Answers 0 Followers

Questions related from Pranav Seth

How much DMEM volume to be added for a 6 Well plate?

Last time I added 2ml per well in a 6 well plate with 400,000 cells per well. This is the normal standard that i have seen in manuals but it gave me a low yield of cells on the day of...

08 August 2018 8,778 0 View

Why does my ponceau staining after the western blot transfer show the lanes to be a little melted towards the end of the running?

I did my gel running and transfer like i always do but this time when i stained the nitocellulose membrane with ponceau , the protein sample lanes apeared different towards the end . They appeared...

06 June 2018 8,546 7 View

I am not able to visualize a certain flavoprotein that I want after my Western blot is complete. My electrophoresis and transfer happen properly?

Hello all, I have been doing Western blotting now for sometime. I make cell lysates from my transfected cells which express a certain flavoprotein. My electrophoresis and gel transfer happens...

01 January 2018 3,116 0 View

I developed my Western Blot today to find that it had no bands. Any idea why?

I could see bands while staining with Ponceau. The antibody solutions and the incubation times are as per requirement.

09 September 2017 9,703 0 View

I have aneGFP expressing vector with a CAG promoter .How long does this eGFP expression take invivo?

It is an AAV vector

09 September 2017 2,689 4 View