Pranav Bhaskar

7 Questions 16 Answers 0 Followers

Questions related from Pranav Bhaskar

How to couple BSA to the SiO2 gel for the purification of anti-heptan-BSA conjugate Antibody from the mixture containing anti-BSA Antibody as well?

I have raised an Antibody against BSA-hapten conjugate. Purified the antibodies with Protein A/G column affinity chromatography. Now, I have a mixture of antibodies consisting of anti-BSA Ab and...

11 November 2017 5,515 2 View

How to store geosmin, a highly volatile compound, after breaking the glass ampule?

I have purchased a highly volatile compound known as geosmin. It was contained in a glass ampule. Geosmin was dissolved in dichloromethane (DCM). I made several aliquots of 30Ul in a 1.5ml...

01 January 2017 3,898 4 View

How to open .xms extension file generated by varian GC-MS system on another PC?

Hello, I got my samples done on Varian GC-MS and it generated .xms extension files. I want to analyze my GC-MS data. How can I open it on my PC? Can anyone suggest me any Mass analyzer software...

12 December 2016 2,288 3 View

What can be done to save the terpenoid product which is highly volatile to evaporate during sample extraction, drying and concetration?

I am trying to set up a reaction by incubating linear isoprenoid precursor Farnesyl diphosphate with the recombinant enzyme in 50mM Tris-Cl (pH 8.2) buffer containing 20% v/v glycerol, 2 mM MgCl2...

10 October 2016 438 3 View

How to determine volatile organic compounds (VOCs) dissolved in Methanol by Thin layer chromatography technique?

I have purchased linear isoprene farnesyl pyrophosphate (as ammonium salt, dissolved in ammonia: methanol) and a bicyclic non-aromatic alcohol geosmin (dissolved in methanol). I am trying to find...

07 July 2016 5,936 3 View

How to check whether the cloning is in-frame or not?

I have cloned my gene in pET28(a) vector and transformed the recombinant plasmid into E. coli BL21 (DE3) and also in E. coli JM109 (DE3). I have checked the putativeness of the clones to be...

08 August 2014 8,061 9 View

For how long can the PCR amplified products be kept at -20 degree centigrade?

I want to know, if I keep PCR amplified products at -20 degree centigrade for overnight, and then I further process it before bacterial cloning. Will it affect the cloning of the bacteria? What...

07 July 2014 4,761 10 View