Prakriti Kalra

7 Questions 8 Answers 0 Followers

Questions related from Prakriti Kalra

Fraction collector Frac-920 not working with AKTA?

Hi, I routinely use AKTA connected to Fraction Collector-920 for collecting proteins while doing protein purification. However, recently I have started seeing some errors on our Fraction...

02 February 2020 2,893 3 View

No colonies from NEB high-efficiency chemically competent cells?

Hi all, I am doing a few site-directed mutagenesis reactions and after digestion of the parent plasmid using Dpn1, I add the complete 50 uL reaction volume to 50 uL of NEB chemically competent...

11 November 2019 8,919 3 View

Synthesis of peptides rich in serines?

Hi all, I am working on the synthesis of a 21 amino acid residues long peptide with the following sequence- NH2SSSDSSSDSSSDSDSSTDDSY-CONH2. The peptide has mutliple serines and a few aspartate...

09 September 2019 7,477 7 View

CD experiments in HEPES buffer?

Hi all, I need to perform CD experiments on my protein both temperature and wavelength scan. My protein is in 50 mM HEPES, 100 mM NaCl pH 7.4 buffer. I have previously acquired some data by...

09 September 2019 3,933 3 View

Low plasmid miniprep yields?

Hi all, I have a few questions regarding the plasmid yields after miniprep. My vector backbone is a pET vector which is low copy number plasmid. I have been getting low plasmid miniprep yields...

06 June 2019 5,778 5 View

No protein expression from Genscript plasmids?

Hi everyone, I have recently ordered a plasmid from Genscript. The vector backbone is pET 28a vector and the gene inserted has been previously reported to yield soluble protein. However, I am...

06 June 2019 8,344 4 View

Low plasmid miniprep yields?

Hi, I have been working on plasmid minipreps using Qiagen miniprep kit and I have run into weird problems of low plasmid yields. When we purchased a new kit, then my plasmid concentrations were...

03 March 2019 9,692 3 View