Prahlad Raninga

4 Questions 11 Answers 0 Followers

Questions related from Prahlad Raninga

Does the size of multiple myeloma colonies on methlycellulose media increase if the cells are drug-resistant?

I am doing colony formation assay using methlycellulose media on RPMI8226 and U266 cells. In the treated samples, number of colonies is reduced compared to the control samples but size of the...

02 February 2015 2,232 5 View

Can anyone suggest a way to detect Bcl-xL, cIAP-1, and survivin in MM cell lines, RPMI 8226 and U266?

I am unable to detect Bcl-xL, survivin, and cIAP-1 protein levels in RPMI8226 and U266 cells. I used Santa Cruz antibodies in 1 in 1000 dilution. I treated cells with a drug for 24 hours, prepared...

01 January 2015 5,639 7 View

Can anyone please suggest a way to transfect plasmid DNAs into the U266 cell line?

I am trying to transfect plasmid DNAs into the U266 cells. I tried Amaxa electroporation, Lipofectamine, and ViaFect. None of these techniques is working. Can anyone please suggest a better way to...

07 July 2014 8,247 2 View

What is the best way to culture myeloma cell lines?

I am currently working on multiple myeloma cell lines. The problem I am experiencing is that these cells are no longer functional after 3 weeks in culture. They are still viable but not functional...

01 January 2014 1,668 15 View