Prafull Kumar Singh

7 Questions 57 Answers 0 Followers

Questions related from Prafull Kumar Singh

Transient expression of a gene by using just the retroviral transfer plasmid possible?

If I transfect my cells just with the retroviral transfer plasmid ( pWZL- Hygro) without the packaging vector (pCl-eco), would it only be transiently expressed? The retroviral vector sequence...

02 February 2020 7,965 3 View

Which is a more biologically relevant loss of function system: Knockdown or Knockout?

Hi everyone With the advent of CRISPR technology knocking out of genes has become a fairly easy and a week job. However I wonder to cope up with the permanent loss of vital genes the cells might...

05 May 2016 900 4 View

Are there Prestained Marker available for Native Gel to enable monitoring during electrophoresis?

Hi Everyone I am doing Native Gel electrophoresis for quite some time and have been using own made protein standards to get an idea about the size of my target proteins. These markers are...

04 April 2016 1,456 4 View

What is the difference between absorption and Excitation spectra?

I am performing cellular uptake studies with peptides labelled with fluorochromes. I did flow cytometry experiments and now I want to validate it by fluorimetry by measuring the emission spectrum...

03 March 2014 2,833 5 View

Does anyone have experience with competition assay with labeled and unlabeled peptide?

I am working on development of cell type specific delivery peptides. To prove the receptor specificity of these peptides, I am performing competition assay between Fluorochrome labelled and...

02 February 2014 4,196 9 View

Black 96 well plates for spectrofluorometry?

I am planning an experiment to quantify the cellular uptake of peptides in different cell lines by fluorometry. I am referring to a paper from Nature Protocols. They recommended using black 96...

02 February 2014 2,187 4 View

Any insight as to Flow Cytometry vs Fluorescence Microscopy?

I am performing experiments for cellular binding of specific peptides. These peptides are labelled with FITC. For some peptides, I do not see any fluorescence in the microscope, however and flow...

02 February 2014 8,395 2 View