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Questions related from Prabuddha Sarkar
Can an eukaryotic gene have slightly long 5' UTR than its 3' UTR? I have one case in my hand, where I found out an ORF containing slightly larger 5' UTR than 3' UTR. And the gene is I think not...
06 June 2013 5,773 10 View
I am facing problem with cleavage of a protein using hydroxylamine. I used guanidine HCl and K2CO3 and then hydroxylamine pH 9 with a 45°C temperature for 5 hrs. After the reaction I got the...
05 May 2013 9,008 4 View
Hi everybody, can anybody tell the maximum size of DNA, which can be inserted in pTZ57R and pFL61? I have a 3.1 Kb fragment amplified from genomic DNA, I want to clone that..
04 April 2012 672 26 View
I have amplified one 3.1 Kb of fragment from genomic DNA, by using primers designed from the 5' and 3' UTR regions from the gene sequence to find out the introns in it. I have checked with the...
04 April 2012 909 39 View
I am facing big problems. I have got the full length gene sequence for my gene. Its 2500bp long approx. I have done 3 RACE s and got the full length by matching overlapping regions. Now I want to...
12 December 2011 4,796 15 View
Hi everybody I am new to this forum. I am having serious problems in doing 5' RACE. I have started to do 5' RACE after getting the 3' end of the gene as I have already mentioned in my other...
06 June 2010 4,300 19 View
