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Questions related from Prabesh Khatiwada
I am currently using a bench top Sonicater at 30% amplitude for 20 cycles (15 sec on, 45 sec off). I am getting chromatin fragments around ~500bp which works for our ChIP-qPCR, but despite trying...
06 October 2020 4,755 1 View
I am searching for papers about mutation to produce kinase active variant of Tec protein. I can see a lot of papers with kinase dead Tec, but I haven't been able to find one with Kinase active.
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I am planning to do SNP analysis in Beta Haemoglobin gene of Nepali planning. We are doing Conventional (RFLP) approach as well as well as AS-PCR. Can anyone suggest me suitable Allele specific...
30 March 2015 1,195 3 View
I am following a procedure for a beta-Glucosidase assay using pNPG. The reaciton mixture of 250 µL of pNPG (1mM), with 50 µL of crude enzyme and 700 µL of sodium acetate buffer (PH 4.8, 50mM) is...
06 May 2013 8,881 4 View
We are planning for the Molecular Identification of Bacterial and Fungal Isolates, identified form various samples/sources. While, for Bacterial 16s rRNA gene, there are universal primers that can...
07 April 2013 6,775 4 View
What are the benefits of Sonication vs Enzymatic digestion (or vice versa) for ChIP and ChIP seq? We are planning to send Prostate cancer cell line samples for ChIP-seq, and getting chromatin...
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