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Questions related from Prabesh Khatiwada
I am currently using a bench top Sonicater at 30% amplitude for 20 cycles (15 sec on, 45 sec off). I am getting chromatin fragments around ~500bp which works for our ChIP-qPCR, but despite trying...
06 October 2020 4,752 1 View
I am searching for papers about mutation to produce kinase active variant of Tec protein. I can see a lot of papers with kinase dead Tec, but I haven't been able to find one with Kinase active.
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I am planning to do SNP analysis in Beta Haemoglobin gene of Nepali planning. We are doing Conventional (RFLP) approach as well as well as AS-PCR. Can anyone suggest me suitable Allele specific...
30 March 2015 1,189 3 View
I am following a procedure for a beta-Glucosidase assay using pNPG. The reaciton mixture of 250 µL of pNPG (1mM), with 50 µL of crude enzyme and 700 µL of sodium acetate buffer (PH 4.8, 50mM) is...
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We are planning for the Molecular Identification of Bacterial and Fungal Isolates, identified form various samples/sources. While, for Bacterial 16s rRNA gene, there are universal primers that can...
07 April 2013 6,773 4 View
What are the benefits of Sonication vs Enzymatic digestion (or vice versa) for ChIP and ChIP seq? We are planning to send Prostate cancer cell line samples for ChIP-seq, and getting chromatin...
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