Pooja Makhija

5 Questions 6 Answers 0 Followers

Questions related from Pooja Makhija

How to induce lipopolysaccharide induced inflammatory damage in HaCaT cells?

i have tried various doses from reported literature such as 12 mcg/mL for 12 h. i have moved to 12-48 mcg/ml for 24 h. No effect on cell viability via MTT is seen. I am using LPS from E.coli.

04 January 2021 667 2 View

Is there any standardized protocol for semi-dry transfer of 12% gel on pVDF membrane (biorad) using transblot system??

I see some of the ladder remaining on the gels at the low molecular weight side, so that raises my concerns.

21 June 2019 4,317 1 View

Can someone let me know if my cell cycle graphs are correct?

I am studying anticancer effect of plant extract on A549 cells using PI staining and BD LSR Fortessa flow cytometer. attached below is the image

30 April 2019 6,236 10 View

Why A549 cells show resistance on reaching p15 passages or more?

A549 cells around P5 to P10 passage were showing very good apoptotic trend in time and dose dependent manner with my plant extract in one set of data. When i tried to replicate data, in the...

23 April 2019 1,646 2 View

How to determine solubility of a dried extract from plant material?

By extract here I mean hexane, dichloromethane, Tetrahydrofuran, methanol, water extracts of a known plant material

12 March 2018 5,218 1 View