Piotr Janas

4 Questions 3 Answers 0 Followers

Questions related from Piotr Janas

LHC-9 media precipitation?

I am looking for advice as what to do with LHC-9 media that contains some kind of precipitate. Gibco is not being very helpful in that matter. My LHC-9 bottles (both sealed and unsealed) contain...

11 November 2018 7,049 0 View

How epigenome changes after passaging immortalized cell line for extended periods of time?

Hi. Could anyone please suggest any literature on the changes in epigenome after cells are cultured for extended periods of time? I had trouble finding anything on immortalized cell lines (there...

09 September 2018 4,759 0 View

Can you conjugate antibodies to MACS magnetic beads (MILITENYI) before the incubation with with cells?

I need to shorten my MACS cell isolation protocol as much as I can. When I perform positive selection for EpCAM+ve cells, the militenyi manual says to incubate cells with EpCAM-PE antibody first,...

06 June 2018 7,016 4 View

I am trying to isolate and culture mouse lung epithelial cells. I don't know what to look for under light microscope. Can you share some photos?

I isolate mouse lung epithelial cells. I can see varius morphologies under the light microscope (the purity is ~90% as assesed by flow cytometry for EpCAM+, CD45-ve, CD31-ve population) presumably...

05 May 2018 8,414 1 View