Pierre Coulon

3 Questions 3 Answers 0 Followers

Questions related from Pierre Coulon

FSC vs SSC. Is SSC dependent on the size too ?

Hello, I have a technical question regarding the measure of the SSC. Following some treatment, my cells are becoming bigger (I can see it with the microscope) and by FACS the FSC increase,...

25 May 2022 1,334 0 View

Is it possible to perform multiple alignement while keeping one specific sequence (only one) gap-free ?

Hi everyone, A naive question probably as I am not an expert in multiple alignement. I would like to know if it is possible to align multiple proteins, allowing gaps in all of the sequence to be...

26 October 2021 6,257 1 View

What could be responsible for this protein degradation/disappearance AFTER serum addition (in Macrophages) ?

Hi everyone ! I have a protein of interest that "disappears" following combination of Starvation then SERUM refeed. Here is the protocol I follow : I starve my macrophages cells OVN in RPMI...

01 January 1970 2,647 0 View