Phoebe Nguyen

5 Questions 8 Answers 0 Followers

Questions related from Phoebe Nguyen

Hello. Can anyone please give me some help about how to determine the amount of enzyme in enzyme assay?

Hello everyone. I am doing an experiment relates to enzyme alpha glucosidase. I extracted this enzyme from 7 strains of thermophilic bacteria from a hot spring. Now I test the enzyme activity of...

01 April 2018 5,658 2 View

Production of secondary compounds in plant tissue culture.

Hello! I would love to know about the production of secondary compounds, especially about phenolic compounds which are produced in the process of culturing plant tissue. It is supposed to be...

28 June 2017 8,032 10 View

Why do we need add beta-mercaptoethanol in sample buffer to determine Bromelain's MW in SDS-PAGE?

When I do the experiment to determine the molecular weight of Bromelain by SDS-PAGE electrophoresis, my teacher ask me to add beta-mercaptoethanol into sample buffer. The role...

01 May 2017 3,427 20 View

Kunitz method - protease assay. Roles of TCA, Cysteine ?

Can anyone help me explain more about the roles of TCA (Trichloroacetic Acid Reagent) and Cysteine in Kunitz method - protease assay. What type of inhibition mechanism of TCA on Casein and why we...

26 April 2017 5,930 7 View

How can we isolate some bacteria strains with low population?

There are some bacteria strains that can tolerate the toxic orgainic solvent, but the number (or population) of them is very low. How can I isolate them? (The bacteria come from soil). Can anyone...

15 April 2017 5,338 4 View