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Questions related from Philip Samaan
I am performing an ELISA to quantify TNFa and perforin in PBMC culture supernatant after 48 hour stimulation with peptide. It's my first time performing the assay, so I lack speed and experience....
02 September 2023 1,485 1 View
My two intependent variables are IFN-y and IL-2 T-cell responses to a given antigen. My dependent variables are 10 different antibody titers for that antigen in serum and saliva. I want to analyze...
22 July 2021 4,044 2 View
For my research project, I am in quite a bit of a dilemma where I need to store whole blood overnight before processing it for PBMC isolation. In order to minimize granulocyte contamination, I am...
28 February 2021 3,089 6 View
Hello all, I am in a bit of a dilemma where I receive blood samples from 6+ patients during the evening. I do not have the energy to process this much blood on my own during the evening, and so I...
14 February 2021 879 5 View
I have been running ELISPOTS with not much success recently due to high levels of background. After testing everything from the DMSO used in peptide stimulation to the FBS in the culturing medium,...
16 October 2020 1,059 3 View
