Philip Samaan

3 Questions 3 Answers 0 Followers

Questions related from Philip Samaan

Addressing Granulocyte Contamination in Cryopreserved PBMC Samples?

For my research project, I am in quite a bit of a dilemma where I need to store whole blood overnight before processing it for PBMC isolation. In order to minimize granulocyte contamination, I am...

28 February 2021 3,083 6 View

What is the best way to store whole blood in ACD tubes overnight prior to PBMC isolation?

Hello all, I am in a bit of a dilemma where I receive blood samples from 6+ patients during the evening. I do not have the energy to process this much blood on my own during the evening, and so I...

14 February 2021 874 5 View

How long can PBMCs survive outside of an incubator in R-10 culturing medium?

I have been running ELISPOTS with not much success recently due to high levels of background. After testing everything from the DMSO used in peptide stimulation to the FBS in the culturing medium,...

16 October 2020 1,031 3 View