Philip Chu

4 Questions 5 Answers 0 Followers

Questions related from Philip Chu

Am i correctly measuring the liquid junction potential in patch clamp?

I corrected for the LJP and got around 20-25 mV, but when I look at other papers this seems high. I corrected for the LJP in a patch clamp rig by leaving external solution in bath and using a...

26 October 2016 3,387 5 View

How do I stop electrode drifting in a patch clamp rig?

Immediately after moving the electrode under the objective, the electrode drifts ~50um (x,y, upwards in the z) and then slows down. after 10 min, the drift is slow but constant (~1um/ minute). We...

30 September 2014 5,121 19 View

Can patch electrode geometry affect rise and decay slopes?

Switched out the filament on our puller and now the patch electrodes appear different and my new recordings have different decay slopes then before. Its the only thing that has changed.

19 March 2014 5,759 26 View

How can I find and target fast spiking cells in the cortex?

I've been using IR-DIC in P32 mouse slice to target cells that appear larger then pyramidal cells that are not pyramidal shaped, or have a different orientation than pyramidal cells. But I've...

06 February 2014 6,486 3 View