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I'm sure we all here know the basics of chromatography, but what are the nifty tips and tricks that you have encountered over the years to aid in your method development? What modifiers (e.g....
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I have a new molecule for which I have initially developed an isocratic LC-MS/MS method (Synergi Fusion column, H2O/ACN 20/80 (+0.1% HCOOH)). Analysis of this method gives limited sensitivity...
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