Pham Ngoc Dung

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Questions related from Pham Ngoc Dung

Why no significant similarity found for a specific DNA sequence using Blastn?

Hi Everyone, I am working on finding the DNA sequence of the proteins with specific function. So far, I got around 5 proteins sequences which show a high similarity (> 96%). I use NCBI to...

22 October 2018 956 3 View

Can centrifuge in gradient density method separate the microplastics from flocs?

Hi everyone, I am working on how to separate microplastics (size = 100 um, density = 1.07 mg/ml) from flocs. I used sucrose 60% w/w (density= 1.28) as a medium in gradient density method to...

18 October 2018 7,988 5 View

Does the abiotic degradation of sulfamethoxazole (SMX) affect to the isolation of SMX-degrading species?

Hi everyone, I have some problem with my bacterial isolation process. I have cultivated SMX-degrading bacteria with contaminated activated sludge as an inoculum for a half of year. The...

05 June 2018 9,308 0 View

MIC test of specific compounds to the growth of antibiotic resistance bacteria?

Hi everyone, I am working on finding minimal inhibitory concentration of a target compound to E. coli harboring resistance plasmid. MICs were determined using turbidity of bacterial culture. The...

05 April 2018 9,525 5 View

How do I calculate relative abundance of a 16SDNA clone library?

Hi everyone, I have one question related to Taxonomical assignment and relative abundance calculation . In order to calculate relative abundance, will I use RDP classifier tool (Wang et al., 2007)...

07 January 2015 277 2 View