Peyman Bakhshaei

6 Questions 3 Answers 0 Followers

Questions related from Peyman Bakhshaei

What is pattern of cytokines after immunization with an acellular vaccine and pertussis aerosol infection in brochoalveolar lavage(BAL) in mice?

Hello, I immunized my mice with an acellular vaccine then I challenged them with Bordetella pertussis by aerosol rout at 2 weeks after second immunization. I sacrificed them and collected their...

12 December 2016 6,538 2 View

Is there any difference between pattern of infection of inbred and outbred mice in challenge with pertussis?

I bought some mice but I wasn't sure that they are inbred. mice were challenged with pertussis and after 18 days were sacrificed.Their BALs were cultured on BG but my control group which weren't...

01 January 2015 9,635 2 View

What is the most accurate method of determining protein concentration?

Except ELISA, because I don't have standard for my recombinant protein.

05 May 2014 848 7 View

Does urea (low concentration) have adverse effects on proteins in tests?

I want carry out a dialysis of my proteins with a buffer containing urea in order to inhibit aggregation.

05 May 2014 4,449 0 View

Is the tetrazolium violet color specific for the BPH1 cell line or is it used for other cell lines?

I'd like a specific color to detect a BPH1 cell line.

04 April 2014 3,484 1 View

Does glycerol have adverse effects in tests for Ex: ELISA, western blot?

If protein is mixed with glycerol...

03 March 2014 5,698 5 View