Peter Molek

3 Questions 7 Answers 0 Followers

Questions related from Peter Molek

Any suggestions for an optimal FRET donor/acceptor dye pair for detecting protein-protein interactions on a solid support?

Hello everybody, I would need some tips regarding the selection of an optimal FRET donor/acceptor (D/A) pair for labeling proteins site-specifically and detect their potential interaction via...

10 December 2015 7,503 5 View

Which part of SV40-ori is sufficient for optimal replication after transferring to another vector?

Hello, does anyone have any experiences with transferring the SV40 origin as independent functional element to another vector/plasmid? I would like to transfer SV40-ori from the pEGFP-N2 plasmid...

12 May 2015 7,796 1 View

Any suggestions with problems on site-directed mutagenesis using QuikChange II Site-Directed Mutagenesis Kit?

Hello, Have anyone a helpful suggestion regarding my problem with site-directed mutagenesis? I wanted to change a single base pair in bacteriophage genomic DNA (the template is a mixture of a...

29 January 2015 509 9 View