Pengfei Ding

3 Questions 15 Answers 0 Followers

Questions related from Pengfei Ding

How to stain RNA for EM?

Does any one know a good reagent (or protocol) to stain nucleic acids (RNA) for electron microscopy (either negative stain or positive stain)? p.s. This is for single particle analysis. Thanks!

11 November 2017 3,628 4 View

Can I add high concentraion NaCl into my GST buffer?

I am using GST-affinity tag to purify my protein. The standard protocol inculdes ~150mM NaCl in the GST buffer. In order to remove the DNA (maybe interact with my protein), I want to increase the...

07 July 2013 3,978 9 View

Which spectrometer should I use to monitor Protein-DNA interaction?

I want to use fluorescent anisotropy(polarization) to detect protein-DNA binding. The signal of tryptophan is different with or without DNA binding. I know that PTI QuantaMaster spectrometer can...

07 July 2013 9,407 7 View