Peng-Yuan Chen

4 Questions 18 Answers 0 Followers

Questions related from Peng-Yuan Chen

What should I do when denaturing a protein solution that whitens throughout the days?

As title mentioned, I have done separation by centrifugation right after the mechanical lysis since I knew the protein of interest was chiefly appeared as inclusion bodies. This was my first time...

05 March 2019 367 9 View

A Cnidarian protein from human cell lines. Are there any suggestions that we should take notice of? Which alternatives can we do?

Hi everyone, Recently I am helping someone to obtain 2 proteins (both are glycohydrolases) from a Hydra species by using Expi293 suspension cells via secretion. He had tried once and they'd...

03 March 2018 5,983 3 View

Why did my values keep jumping around when increasing ligands? Are there any limitations when doing the FP assay?

It's my first time to do FP assay. I help measure Kds of inhibitors. I got two probes (hereafter P1 and P2, both containing FITC) and one protein sample (Pr). Kd(Pr, P1) is around 30 nM (done by...

15 August 2016 1,054 3 View

Does everyone use the universal units for Vmax in enzyme assays?

Recently I've been training a first-year PhD student to do enzyme assays. The problem is the notations for the parameters of the results, particularly Vmax. The data of the assays we did are in...

01 January 1970 8,496 19 View