Why am I not seeing LecA from Pseudomonas aeruginosa extracts?

I am trying to detect LecA from a Pseudomonas aeruginosa PAO1 extract, for which I do a liquid culture overnight in LB broth (37oC, 200 rpm), harvest bacteria (10^8 cells) and lyse in presence of...

28 May 2022 2,404 2 View