Paweł Nowialis

3 Questions 2 Answers 0 Followers

Questions related from Paweł Nowialis

Are there any caveats in performing a Sequential ChIP (Re-ChIP) experiments using antibodies against two different epitope tags?

We are trying to design an experiment investigating chromatin co-localization of two DNA binding proteins in the system where both endogenous genes are knock-out and re-expressed with attached...

11 January 2021 3,895 2 View

What is the consequence of DRP1 phosphorylation on mitochondrial dynamics?

Reading literature about mitochondria fission/fusion mechanism I came across two oposite explanations on role of phosphorylation of DRP1 in regulation of mitofission. Study of Han and colleagues...

28 August 2015 8,474 2 View

How may I improve double IF staining of white and brown adipose tissue?

I am trying double IF staining of PARKIN and COX IV on PFA section or Frozen sections of inguinal white adipose tissue and interscapular brown adipose tissue. Working with the paraffin sections I...

22 July 2015 6,957 4 View