Pavlina Cejkova

5 Questions 3 Answers 0 Followers

Questions related from Pavlina Cejkova

QPCR: What can be the reason for lowest Ct values in the lowest concentrated samples?

Hi, I ran a standard curve on several DNA samples to determine the efficiency of PCR. However, I noticed a strange thing: the first three concentrations worked quite well, while the last two...

29 November 2022 6,525 5 View

How to improve the extremely low 260/230 ratios and yields of DNA isolated from teeth?

Hi, I have isolated DNA from the 50 mg teeth ground to a powder by using the QIAamp DNA investigator kit (since it contains the protocol devoted to isolation from this type of tissue). I strictly...

18 February 2022 872 4 View

QPCR data analysis with different efficiencies in GOI and HK and no calibrator?

I am facing the troubles with relative QPCR data evaluation. I have two groups - patients vs. healthy controls. Usually I use 2^-dCt method and compare by Student t-test or Mann-Whitney test the...

04 June 2017 2,558 1 View

THP1 cell line to share?

Hi, I am looking for suitable cell line model for my new experiment and THP-1 is one of my choice to test. If someone has these cells cultured under ATCC-recommended condition (without additional...

28 June 2016 4,461 1 View

Is it possible to fix cells first and then stain for membrane and intracellular antigens (FCM)?

I wonder if possible to fix your cells first prior to both, membrane antigen and IC staining. All protocols available suggest to stain for membrane markers, fix, permeabilize and finally stain for...

14 May 2015 3,640 4 View