Pavel Nash

2 Questions 2 Answers 0 Followers

Questions related from Pavel Nash

Can you provide me with an efficient plasmid extraction protocol from yeast?

I've been trying to isolate a low copy (CEN/ARS) plasmid from yeast that has selective markers for both yeast and bacteria (Trp and Amp). However, when I try to recover the plasmid I can't get any...

21 October 2023 6,702 2 View

How do I improve my lentiviral transduction efficiency with fibroblasts?

I have measured my physical titer of my lentiviral genome copies to be over 10^10, when taking an aliquot out of liquid N2. Despite this, when I treat fibroblasts with my lentiviral prep I have a...

29 April 2019 5,793 4 View