Paulo César Martins Urbano

6 Questions 10 Answers 0 Followers

Questions related from Paulo César Martins Urbano

Does any one know a good caspase 3/7 dye that remain in the cell after wash?

I´m looking at organoids and T cell co-culture, and I´d like to know if T cells can kill organoids. The idea is to label organoids with caspase 3/7 dye (in theory, it will be internalized by...

22 September 2022 9,248 0 View

Does anyone have experience on ImageXpress Micro confocal from Molecular Device?

We have bought the ImageXpress Micro Confocal High-Content Imaging System from molecular device but we're struggling with the MetaXpress software. Could anyone be welling to help us to run a...

02 September 2022 5,565 0 View

How to grow Colorectal Cancer Organoids from biopsies?

I´m having some trouble growing CRC organoids from biopsies (maybe with low tumor cells present). Is there any trick that could improve the growth of CRC organoids from biopsies? Material and...

13 January 2022 7,589 3 View

What is the best approach to measure leakage of 3D intestinal organoids embedded in matrigel?

I'm working with intestinal organoids (colonoids) and I wonder what is the best approach to measure intestinal leakage. I want to measure inside of the matrigel, without transferring them to...

12 August 2020 3,983 2 View

How do I reduce basal phosphorylation in PBMCs for phosphoFlow detection?

I'm measuring the phosphorylation status of different proteins (e.g. NFKB, ERK, AKT1, p38 etc) using phosphoflow cytometry. The protocol for surface and intracellular staining works well, all the...

23 July 2020 3,394 8 View

Postdoc position in intestinal organoids (translation medicine) - Denmark

Dear all, Please reach out if you´re PhD and have worked with intestinal organoids. Our lab in Denmark is looking for a post-doc to continue our drug screening platform using organoids generated...

01 January 1970 2,412 0 View