Paula Gonzalez

3 Questions 2 Answers 0 Followers

Questions related from Paula Gonzalez

Can someone reccomend a lentiviral backbone for microRNA sponge in vivo delivery?

Hello, I'm considering using microRNA sponges for an in Vivo experiment and I'm looking for lentiviral backbones where I could potentially clone the sponge. Since I want to use this system in...

24 July 2020 5,569 0 View

Has anybody experience in brain in utero electroporation using ISOLATED PULSE STIMULATOR MODEL 2100?

Hello, I want to do KO using Crispr plasmids in mice embryos during development. I would like to electroporate E12.5 embryos, and in the lab, the electroporator that is available is the ISOLATED...

14 February 2020 9,794 0 View

EO771 mouse tumor transfection protocol?

Hello! I've been trying to transfect EO771 using lipofectamine 2000, but despite changing the transfection media after 6 hours of incubation I have a high rate of cell death. My untransfected...

14 November 2019 8,015 3 View