Paul Taslimi

4 Questions 5 Answers 0 Followers

Questions related from Paul Taslimi

Noise in ADP Glo assay?

I am using the Promega ADP Glo assay to look at inhibitors. I am getting a very large amount of noise in the assay. The noise is so bad that I cant reliably measure compound IC50s. I wonder if...

03 March 2019 8,926 2 View

Detergent effect on inhibitors?

I wonder if anyone has seen the potency of inhibitors increase in the presence of detergent. I included 0.01% Triton in an assay which previously was not giving any hits and now I see a few! I...

11 November 2018 3,728 2 View

How do Arrhenius plots change in the presence of enzyme activators or inhibitors?

Does the slope chg, or some other parameter?

11 November 2017 3,828 6 View

How does enhancement of fluorescence at the excitation wavelength occur?

I am looking at an NBD labeled lipid. I see a large enhancement of the excitation intensity but very little in the emission intensity upon addition of protein. I was wondering how this can occur...

10 October 2017 9,463 3 View