Paul Degregory

3 Questions 3 Answers 0 Followers

Questions related from Paul Degregory

How can sandwich assay dynamic range be expanded to higher concentration ranges, especially for simultaneous mixing of capture and reporter agents?

I've tried increasing the concentration of both capture and reporter agents to increase binding capacity on both ends of the sandwich, however the peak of the 'hook effect' hasn't shifted....

11 November 2014 5,208 2 View

Why does antibody-antigen binding have lower affinity in urine than in buffer?

I'm doing a metalloimmunoassay involving conjugation of an antibody to silver nanoparticles for reporter formation. Long story short, I've gotten the assay to work well, but my signals are 10x...

09 September 2014 3,905 6 View

Why am I having problems with my silver nanoparticle-antibody conjugation for immunoassay?

I'm trying to conjugate an antibody to silver nanoparticles (20 nm diameter, citrate capped AgNPs). The conjugation protocol essentially involves centrifuging AgNP stock, resuspending at the same...

08 August 2014 5,450 2 View