Paul N. Span

5 Questions 11 Answers 0 Followers

Questions related from Paul N. Span

Does anyone have a schematic for hypoxic containers for irradiation experiments?

Does anyone have a schematic for hypoxic containers for irradiation experiments? We want to irradiate cells under hypoxia, and need containers for cell culture flasks that can be closed airtight...

26 January 2024 2,252 1 View

Best method to measure viability of spheroids in Incucyte?

Does anybody have experience with quantifying viability and/or cell death of (breast) cancer spheroids using the Incucyte Zoom system?

20 March 2018 4,337 1 View

Does anyone know of an ACTH receptor (MC2R) positive cell line?

Does anyone know of an ACTH receptor (MC2R) positive cell line? So far I have not been able to find a readily available cell line expression this receptor and that is responsive to ACTH.

21 July 2016 3,260 3 View

How can I authenticate cell lines and validate their origin by comparing with original patients samples?

We have cell lines that were obtained from patient tumors. We would like to validate their origin using a cell line authentication service or similar. Does anyone have experience with this?

08 October 2013 1,279 12 View

Can nuclear EGFR be quantified using flow cytometry?

Does anybody have any experience with quantifying nuclear EGFR (or any other nuclear protein) using FACS analysis? The EGR is normally, and far more abundantly located on the plasma membrane. I am...

23 May 2012 8,087 9 View