Patrycja Ogonowska

2 Questions 4 Answers 0 Followers

Questions related from Patrycja Ogonowska

Could anyone help me with interpreting the results in qPCR method with using relative quantification?

Welcome everyone! In my research I focus on comparing Staphylococcus aureus genes to the reference gene - gmk. In qPCR I’m using relative quantification and the following formula: ∆Ct (target)=...

08 August 2018 8,048 0 View

What is the problem with the analysis in the LightCycler 480? Two-step cycling?

Hi! I have a problem with the analysis in LightCycler 480 Software. I prepared the two-step cycling experiment with the following conditions: 95C 15s, 60C 1 min (see the picture below). I based on...

07 March 2018 6,913 7 View