Patrick Ovando-Roche

3 Questions 10 Answers 0 Followers

Questions related from Patrick Ovando-Roche

Whats the best Amaxa program to transfect H9 hESCs with a large 17kb plasmid?

I was wondering if anyone knows whats the best Amaxa electroporator program to transfect a large 17kb plasmid. I tend to use CB150 which works ok for plasmids up to 12kb. However, I have not...

12 April 2016 4,462 0 View

Can you gel purify promoter+gRNA ONLY (i.e. no backbone) for RNA synthesis (CRISPR Cas9 gene editing)?

gRNA kits say to "linearise" the plasmid containing your promoter+gRNA right after the termination signal. I was wondering whats the point of having the whole backbone there?. Cant you just cut at...

08 May 2015 1,106 3 View

How can I put a blunt-sticky ligation of 6.3kb insert into 2.3kb backbone?

Can anyone give some advice on my cloning? This is probably not the situation that you want to have where your insert is almost 3 times larger than your backbone. I tried several times now. I use...

02 July 2014 8,731 14 View