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Questions related from Patrick Ovando-Roche
gRNA kits say to "linearise" the plasmid containing your promoter+gRNA right after the termination signal. I was wondering whats the point of having the whole backbone there?. Cant you just cut at...
08 May 2015 1,078 3 View
Can anyone give some advice on my cloning? This is probably not the situation that you want to have where your insert is almost 3 times larger than your backbone. I tried several times now. I use...
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