Patricio Orrego

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Questions related from Patricio Orrego

How can I preserve slices of rat hippocampus after stimulating them for LTP, for a subsequent non-immediate analysis by western blot?

I need to have a certain number of treated slices and the respective controls prior to the extraction of respective proteins. What is the best way to freeze the slices and then homogenize each...

12 December 2017 7,703 1 View

What is the best method for breaking cell wall Muriellopsis (microalgae)?

I want to obtain carotenoids. Thanks.

07 July 2016 771 1 View

Any solution for problems with Batch Method Purification of his-tagged-proteins?

I have to purify a protein with 6xHis tag, but at the time of dissociating the protein from the resin (Ni-NTA) all protein remains in the resin. A feature of my protein is that it is rich in...

05 May 2016 4,895 6 View

What is the best way to remove platelets after centrifugation using Ficoll-Paque?

I need to get monocytes from to differentiate into macrophages.

10 October 2015 7,982 7 View