Patricio R. Orrego

4 Questions 2 Answers 0 Followers

Questions related from Patricio R. Orrego

In which days in vitro rat hippocampal neurons have a higher secretion rate?

I need to determine in a trial with hippocampal neurons if an inhibitor affects the secretion of a glycoprotein. What stage (DIV) would be the most appropriate to visualize it ?. Thanks.

12 October 2018 4,670 0 View

Which experimental approach is the most appropriate to determine the hal-life of a protein in the plasma membrane?

The idea is to visualize by microscopy the arrival to the surface of the plasma membrane and to determine the time until the protein is endocyted again. Thanks.

15 March 2018 3,313 2 View

Any solution for problems with Batch Method Purification of his-tagged-proteins?

I have to purify a protein with 6xHis tag, but at the time of dissociating the protein from the resin (Ni-NTA) all protein remains in the resin. A feature of my protein is that it is rich in...

20 May 2016 8,422 6 View

Is necessary to supplement the culture medium with defibrinated plasma for obtain monocytes?

I need isolate monocytes from human peripheral blood and with minimal contamination with platelets. Is really necessary to supplement the means of monocytes with Heat-inactivated (HI) autologous...

28 July 2015 9,903 0 View