Patrícia R. Albuquerque

5 Questions 2 Answers 0 Followers

Questions related from Patrícia R. Albuquerque

Which would be the best control microRNA to normalize exosomal microRNAs expression?

I'm evaluating microRNAs expression in neuronal cells-derived exosomes. I was wondering which microRNA I should use as control to normalize my results. Thanks in advance

05 September 2014 7,582 3 View

Has anyone used CD63 and CD81 antibodies from Santa Cruz Biotec. for identifying exosome isolation?

I'd like to know whether someone has been using CD63 and CD81 antibodies from the brand Santa Cruz Biotec. to confirm exosome isolation through western blotting. I've had some problems and I'd...

23 April 2014 4,372 6 View

Which protocol do you recommend for exosomes isolation from human blood (plasma)?

I'm intending to isolate exosomes from human plasma. I've checked some available commercial kits but I really want to try protocols based in centrifugations, the traditional approach. Any...

03 February 2014 5,453 1 View

What would be the best exosomal mRNA to exclude cellular contamination when performing qPCR?

I've isolated exosomes from a neuronal cell line and I've isolated exosomal RNA and I intend to perform qPCR. So, I was wondering which mRNA could be useful to attest the purity of my exosomal...

03 November 2013 5,389 1 View

What would be the best house keeping gene to study the exosomal mRNA expression?

I'm intending to perform RT-PCR to check the mRNA expression in exosomes but I've some doubts regarding the housekeeping genes that I should use. Thanks in advance

17 October 2013 9,415 4 View