Patrícia Guerreiro

3 Questions 1 Answers 0 Followers

Questions related from Patrícia Guerreiro

Does anyone have a protocol for CD24 detection by confocal microscopy in mammalian cell lines?

I am using confocal microscopy to confirm the flow cytometry results regarding CD24+ cells. The mammalian cell line is expected to be CD24+. as confirmed by our FACS experiments. However, standard...

04 April 2017 1,561 4 View

How to prepare a solution of CMT-3 (or Col-3) to evaluate cell cytotoxicity?

I am trying to evaluate the cytotoxicity of CMT-3 (MMP inhibitor) in cancer cell lines. I have prepared a stock solution in DMSO. Now, I am trying to prepare my work solution in water with a final...

11 March 2015 9,571 1 View

Should the same factor as staining with ethidium bromide be used to perform the quantification of plasmid DNA stained with GelRed?

For the quantification by densitometry of supercoiled and relaxed forms of plasmid DNA stained with ethidium bromide and separated in agarose gel there is a correction factor of 1.4 because the...

12 May 2014 119 2 View