Parisa Moghaddam-Taaheri

4 Questions 4 Answers 0 Followers

Questions related from Parisa Moghaddam-Taaheri

Why does gel electrophoresis of genomic DNA PCR product shows smearing and a lot more DNA than expected?

We did a genomic extraction (Qiagen) on Stachybotrys chartarum and tried to measure the concentration in my eluate using a NanoDrop. I don't believe this is giving me an accurate reading...

02 February 2018 6,582 3 View

Why is a truncated version of my insert being inserted into my vector during molecular cloning?

Hi, I'm having an issue with cloning the full length of my PCR product into my vector. What I have done: 1.) PCR to amplify my insert where primers add 5' NheI and 3' BamHI sites (with 6 extra...

04 April 2017 6,068 7 View

How can I remove DMSO from plastic?

Some DMSO (dimethyl sulfoxide) was spilled on the plastic part of our thermal cycler and when a cycle was ran, it corroded the plastic from the lid to that of the base of the instrument such that...

04 April 2017 7,328 5 View

How can I ensure my E.coli continues to grow even while inducing expression overnight?

I needed to do a culture and subculture all in one day but my cells are growing slowly. I'm growing BL21-DE3 cells transformed with plasmid and started a culture from a freeze into 3 mL LB+Amp for...

04 April 2015 9,040 4 View