Parastoo Hosseini

4 Questions 1 Answers 0 Followers

Questions related from Parastoo Hosseini

Is antigen retrieval used for frozen tissue sections?

Hi, I would be thankful if you could help me with some issue in IF staining. I've fixed some rat brain tissues in PFA (4%) then preserved in sucrose-PBS (30%) for 7-8 months. Does long storage in...

13 May 2023 828 5 View

Should I use parametric or non-parametric method for my test?

Hi everyone, I want to analyze my data for the plantar test. I have 4 groups in my study. 2 groups are related to treatment and 2 are control and vehicle. my data for treatment groups are normal...

26 July 2021 1,885 1 View

How to increase zebrafish survival rate after spinal cord injury ?

Hi everyone I'm having trouble with the high mortality rate of my zebrafish after surgery. ( the surgery is done based on this article: Becker, C. G., Lieberoth, B. C., Morellini, F., Feldner,...

14 September 2020 6,906 1 View

How to isolate transected zebrafish spine for RNA extraction?

hello everyone, I have to isolate the zebrafish spine after spinal cord injury for analyzing multiple genes expression, but that is so delicate, is there any protocol for that? does it matter if I...

14 September 2020 4,149 0 View