Papori Buragohain

2 Questions 6 Answers 0 Followers

Questions related from Papori Buragohain

Can anyone suggest what can be the possible challenges in a SELEX against hydrophobic targets?

I am trying to do a SELEX to find DNA aptamers against few selected hydrophobic peptide targets of length 13-17 mers but not yet able to obtain successful candidates. I tried with different types...

18 January 2018 1,728 3 View

Why does the ITC curve in an interaction between a DNA aptamer and peptide ligand have both exothermic and endothermic peaks at the same time?

I am using MicroCal ITC 200 (GE) and performing an interaction study between a DNA aptamer (12 kDa) and a peptide ligand (1.91 kDa). How can I explain an ITC curve with both negative and positive...

28 December 2017 370 4 View