Any advice to get the correct construct after failure with the gibson assembly kit?

I'm using the gibson assembly to assemble 3 fragments (1.5kb 1,5kb and 3kb) into a vector (topo2.1 4kb) to generate a 10Kb plasmid. 2 fragments and the vector are PCR amplified and purified from...

03 March 2015 8,819 4 View